WHO Hand Sanitizer Formulas

If you need a supply of sanitizer, here are the two WHO approved formulas for manufacture

 These will produce 1 liter of sanitizing rub.

 Formulation I

 To produce final concentrations of ethanol 80% v/v, glycerol 1.45% v/v, hydrogen peroxide (H2O2) 0.125% v/v.

 Pour into a 1000 ml graduated flask:

Ethanol 96% v/v

833.3 ml

(93.7 parts)

Hydrogen Peroxide (H2O2 3%)

  41.7 ml

  (4.7 parts)

Glycerol 98%

  14.5 ml

  (1.6 parts)

 Top up the flask to 1000 ml with distilled water (110.5 ml) or water that has been boiled and cooled; shake the flask gently to mix the content.

 

Formulation II

 To produce final concentrations of isopropyl alcohol 75% v/v, glycerol 1.45% v/v, hydrogen peroxide 0.125% v/v:

 Pour into a 1000 ml graduated flask:

Isopropyl alcohol (with a purity of 99.8%)

751.5 ml

(93.1 parts)

Hydrogen Peroxide (H2O2 3%)

  41.7 ml

  (5.2 parts)

Glycerol 98%

  14.5 ml

  (1.7 parts)

 

Top up the flask to 1000 ml with distilled water (192 ml) or water that has been boiled and cooled; shake the flask gently to mix the content.

 Only pharmacopoeial quality reagents should be used (e.g. The International Pharmacopoeia) and not technical grade products.

 The last time we checked, supplies are still available from various chemical supply houses.

 If you would like to read the complete WHO Guidelines on Hand Hygiene in Health Care, we have a PDF version available for download HERE.

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